Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Continuous plasma filtration coupled with sorbents

C Tetta¹, J M Cavaillon, G Camussi

¹Clinical and Laboratory Research Department, Bellco S.p. A., (Modena), Italy. Tetta.ciro@arcanet.it

Kidney International. Supplement

|May 9, 1998

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

sRAGE: a prognostic factor for mortality in end-stage renal disease patients on dialysis.

Journal of biological regulators and homeostatic agents·2019

Same author

The E3 ubiquitin ligase WWP1 sustains the growth of acute myeloid leukaemia.

Leukemia·2017

Same author

Elevated Levels of Procalcitonin and Interleukin-6 are Linked with Postoperative Complications in Cardiac Surgery.

Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society·2017

Same author

Nonvitamin K-dependent oral anticoagulants (NOACs) in chronic kidney disease patients with atrial fibrillation.

Thrombosis research·2017

Same author

Pathophysiology of the cardio-renal syndromes types 1-5: An uptodate.

Indian heart journal·2017

Same author

The Dose Response Multicentre Investigation on Fluid Assessment (DoReMIFA) in critically ill patients.

Critical care (London, England)·2016

Same journal

Effect of kidney disease on glucose handling (including genetic defects).

Kidney international. Supplement·2011

Same journal

Need for better diabetes treatment for improved renal outcome.

Kidney international. Supplement·2011

Same journal

Glucose dynamics and mechanistic implications of SGLT2 inhibitors in animals and humans.

Kidney international. Supplement·2011

Same journal

SGLT2 inhibitors: molecular design and potential differences in effect.

Kidney international. Supplement·2011

Same journal

Glucose handling by the kidney.

Kidney international. Supplement·2011

Same journal

Bone: from a reservoir of minerals to a regulator of energy metabolism.

Kidney international. Supplement·2011

See all related articles

Synthetic membranes effectively removed inflammatory cytokines and their antagonists from blood in vitro. This approach shows promise for treating sepsis by reducing harmful mediators and potentially improving patient survival.

Area of Science:

Biomedical Engineering
Immunology
Sepsis Research

Background:

Sepsis involves complex immune responses with elevated proinflammatory cytokines and antagonists.
Simultaneous removal of these mediators may mitigate sepsis-induced complications.

Purpose of the Study:

To evaluate an in vitro system for simultaneous removal of cytokines and their antagonists from blood.
To assess the efficacy of different sorbent membranes in this removal process.

Main Methods:

An in vitro system using plasma separation membranes with natural (charcoal) or synthetic (Amberlite, Amberchrome) sorbents.
Whole blood was spiked with bacterial lipopolysaccharide (LPS) to simulate sepsis conditions.
Evaluated clearance of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), IL-8, IL-1 receptor antagonist (IL-1Ra), and soluble TNF-alpha receptors (sTNFR I and II).

Related Experiment Videos

Main Results:

Plasma filtration significantly enhanced the clearance of all tested cytokines compared to ultrafiltration.
Synthetic Amberlite membranes effectively absorbed IL-1Ra, IL-1 beta, and IL-8 (nearly 100%) but showed lower TNF-alpha removal (40%).
Synthetic Amberchrome membranes demonstrated effective TNF-alpha removal (80%).

Conclusions:

Synthetic sorbent membranes show potential for simultaneous removal of key inflammatory mediators in a sepsis model.
This extracorporeal approach may help manage hemodynamic alterations in sepsis.
Further clinical investigation is required to confirm efficacy in human patients.