Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Humanizing the yeast telomerase template

K A Henning1, N Moskowitz, M A Ashlock

  • 1Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892-4442, USA.

Proceedings of the National Academy of Sciences of the United States of America
|May 20, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Correlation between ABO Blood Types and Alcoholic Liver Disease.

Clinical laboratory·2025
Same author

[The status and changing trend in the burden of refractive errors in China from 1990 to 2019].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2024
Same author

[EBF1 Promotes the Sensitivity of Cervical Cancer Cells to Cisplatin via Activating FBN1 Transcription].

Molekuliarnaia biologiia·2023
Same author

Small intestinal morphology and sugar transporters expression when consuming diets of different energy levels: comparison between Tibetan and small-tailed Han sheep.

Animal : an international journal of animal bioscience·2022
Same author

[Eosinophilic solid and cystic renal cell carcinoma with tumor-induced osteomalacia: report of a case].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2021
Same author

Tazarotene gel promotes healing of deep tissue injury in mice.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·2021

Researchers modified yeast telomerase RNA (TLC1) to use the human telomere sequence (TTAGGG)n. This genetic modification resulted in viable yeast strains that add human telomere repeats to their chromosomes, offering new research tools.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Yeast telomeres have irregular (TG1-3)n repeats, unlike human (TTAGGG)n repeats.
  • Telomerase RNA (TLC1) in Saccharomyces cerevisiae encodes the telomeric repeat sequence.

Purpose of the Study:

  • To investigate if altering the TLC1 template can change the telomeric repeat sequence in yeast.
  • To create a yeast model for studying human telomere biology and telomere-binding proteins.

Main Methods:

  • Genetic modification of the Saccharomyces cerevisiae TLC1 gene to encode the human telomere repeat sequence.
  • Creation and analysis of haploid yeast strains expressing the modified TLC1 allele (tlc1-human).
  • Southern hybridization and sequencing of yeast artificial chromosome ends to verify telomere repeat addition.

Related Experiment Videos

Main Results:

  • Haploid yeast strains with the tlc1-human allele are viable and express the gene at wild-type levels.
  • Southern hybridization confirmed the addition of (TTAGGG)n repeats to yeast chromosome ends.
  • Sequencing verified the molecular-level incorporation of human telomeric repeats.

Conclusions:

  • The template sequence of yeast telomerase RNA is responsible for the irregularity of yeast telomere sequences.
  • The tlc1-human yeast strains are valuable tools for studying telomerase function, regulation, and human artificial chromosome propagation.