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X-ray analysis of DNA dodecamer containing 2'-deoxy-N6-methoxyadenosine
T Chatake1, T Toyoda, M Tsunoda
1Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
Oxyamines have been known as a mutagen which attacks amino groups of DNA bases. It is expected that the modified adenine derivative has a tautomer which can form a stable base pair not only with thymine but also with cytosine. For establishing such tautomerization mutagenesis, we have solved a crystal structure of DNA dodecamer containing 2'-deoxy-N6-methoxyadenosine. It is shown that the N6-methoxyadenine takes a imino form to form a Watson-Crick type pairing with cytosine in the DNA duplex.