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New multi zinc finger protein: biosynthetic design and characteristics of DNA recognition
T Kamiuchi1, M Imanishi, E Abe
1Institute for Chemical Research, Kyoto University, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
New six zinc finger protein DSp1 was created by connecting two Sp1 DNA binding domains. The DNA recognition characteristics of DSp1 demonstrate that (1) this multi finger protein binds eighteen contiguous base pairs of DNA sequence, (2) each zinc finger wraps around DNA major groove, and (3) two Sp1 domains have nonequivalent contribution to the DNA binding of DSp1.