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Novel primers designed for microsatellite loci in Eucalyptus and identification by PCR fingerprints
1Biochemistry Section, Iwakuni Research Laboratory of Technology, Nippon Paper Industries Co. Ltd, Yamaguchi, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
We found a novel PCR-primer which can be used for the identification on "elite-tree-selection". This primer was designed for selective hybridization at the both ends of microsatellite loci, which is well known as one of the most highpervariable region of DNA. After PCR-fingerprinting on five Eucalyptus species (E. globulus, E.citriodora, E.grandis, E. maidenii, E.bicostata), with our primer, DNA-polymorphism was observed all over the cases.