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Expression of Xenopus laevis translation initiation factor 4E (eIF-4E) by baculovirus-insect cell system
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
A gene encoding Xenopus laevis eIF-4E was cloned into a transfer vector, and its gene expression was attempted in cells of E. coli, yeast and insect. Effective expression of the active eIF-4E was achieved in the soluble fraction of the insect cell Sf9, which was infected with the recombinant baculovirus. Overexpression of the eIF-4E protein caused remarkable change in the shape of the cells.