Related Experiment Videos
Specific RNA aptamers to NS3 protease domain of hepatitis C virus
K Fukuda1, D Vishinuvardhan, S Sekiya
1Natioal Institute of Bioscience and Human Technology, Tsukuba Science City, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
In order to isolate RNA aptamers that bind specifically to NS3 protease domain (delta NS3) of hepatitis C virus, we carried out in vitro selection procedure using RNA pool that had 30 N random core region. After repeating nine cycles of selections and amplifications, a pool of RNAs that bind specifically to the delta NS3 were selected. A comparative analysis of 45 clones that were isolated from 9th cycle revealed three main classes that contain the conserved loop sequences GANUGGGAC. Moreover, the predominant class of aptamer (class I and III) appear to inhibit the protease activity efficiently.