Related Experiment Videos
Structural analogues of TaqMan probes for real-time quantitative PCR
R G Kuimelis1, K J Livak, B Mullah
1PE Applied Biosystems, Foster City, CA 94404, USA.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
We have prepared and evaluated a series of structural analogues of TaqMan PCR probes in an effort to identify second-generation probes with improved physical properties and performance. Modifications have included non-nucleosidic dye linkers, 2'-O-Me RNA substitutions, and pyrimidine C5-propyne substitutions.