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Updated: Aug 2, 2026
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
Published on: August 2, 2015
Detection, purification and characterization of a protein that binds the (6-4) photoproduct-containing DNA in HeLa
Y Fujiwara1, C Masutani, F Hanaoka
1Department of Bioorganic Chemistry, Biomolecular Engineering Research Institute, Osaka, Japan.
Abstract:
HeLa cell proteins that bind DNA containing the pyrimidine(6-4)pyrimidone photoproduct were detected by the electrophoretic mobility shift assay using synthetic oligonucleotide duplexes as probes. The major species was purified to near homogeneity, and the amino acid sequences of the proteolytic peptides revealed that it was the human damage-specific DNA-binding protein, which was reported previously. The substrate specificity of this protein was determined using damaged or modified DNA duplexes.
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