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Poly(L-lysine)-graft-dextran copolymer is a novel stabilizer of triplex DNA(II): potassium-insensitive triplex
A Ferdous1, H Watanabe, A Maruyama
1Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
Triple helix formation involving guanine-rich oligonucleotides is inhibited by physiological concentrations of potassium (K+). Using in vitro electrophoretic mobility shift assay (EMSA), we show that PLL-g-dex copolymer significantly stabilizes triplex structure at physiological levels of K+ that greatly inhibited triplex formation with a unmodified, guanine-rich oligonucleotide.