Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A protein binding specifically to the IgG2b switch region

H Völk1, M Wabl

  • 1Department of Microbiology and Immunology, University of California, San Francisco 94143-0670, USA.

Developmental Immunology
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum anti-GM2 and anti-GalNAc-GD1a IgG antibodies are biomarkers for acute canine polyradiculoneuritis.

The Journal of small animal practice·2021
Same author

Corpus callosal abnormalities in dogs.

Journal of veterinary internal medicine·2014
Same author

Cervical vertebral stenosis associated with a vertebral arch anomaly in the Basset Hound.

Journal of veterinary internal medicine·2012
Same author

Foreign body induced neuritis masquerading as a canine brachial plexus nerve sheath tumour.

Veterinary and comparative orthopaedics and traumatology : V.C.O.T·2009
Same author

The possibility of B-cell-dependent T-cell development.

Scandinavian journal of immunology·2003
Same author

IL-10 and its homologs: important immune mediators and emerging immunotherapeutic targets.

Trends in immunology·2001

Researchers identified a protein that binds specifically to the S gamma 2b DNA sequence in Abelson-virus-transformed B-cells. This binding factor is likely a component of the switch recombinase, crucial for immunoglobulin class switching.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • The Abelson-virus-transformed mouse pre-B-cell line 18-81 exhibits exclusive immunoglobulin class switching from mu to gamma 2b.
  • Understanding the molecular mechanisms of immunoglobulin class switch recombination (CSR) is vital for immune system function.

Purpose of the Study:

  • To isolate and characterize a nuclear factor that specifically binds to the S gamma 2b switch region.
  • To determine if this factor plays a role in the CSR process.

Main Methods:

  • Isolation of nuclear extracts from the 18-81 cell line.
  • An eight-step purification process employing DNA-affinity chromatography.
  • Gel shift assays to analyze protein-DNA binding specificity.

Related Experiment Videos

Main Results:

  • A protein factor specifically binding to S gamma 2b was isolated.
  • Purification yielded a preparation with two distinct protein bands around 69 kD.
  • Gel shift assays confirmed specific binding to S gamma 2b, with S mu unable to compete.

Conclusions:

  • The isolated factor specifically recognizes and binds to the S gamma 2b DNA sequence.
  • This protein is a strong candidate for being part of the switch recombinase complex involved in CSR.