[Quantitative analyses of DNA content and P53 gene product expression from epithelial lacrimal gland tumors]
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Updated: Oct 29, 2026
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
1Department of Ophthalmology, Beijing Institute of Ophthalmology, Beijing Institute of Ophthalmology.
Objective:
To investigate the significance of quantitative analyses of DNA content and P53 gene product expression which are applied for pathological diagnosis of epithelial lacrimal gland tumor.
Methods:
Flow cytometry (FCM) and immunofluorescence staining technique were used to quantitatively measure the DNA content and expression of P53 gene product from 39 cases with epithelial lacrimal gland tumors.
Results:
It is indicated that the pleomorphic adenomas still possess of the properties of DNA diploid of normal lacrimal gland cell, but malignant tumors are characterized by DNA aneuploidy. There is a significant difference in the content of expression of P53 gene product between pleomorphic adenomas and malignant tumors (P < 0.001). The content of expression of P53 gene product is positively correlated to the DNA content, and both are increasing with the reduction of tumor tissue differentiation.
Conclusion:
Quantitative analyses of DNA content and expression of P53 gene product may provide objective indexes for the pathological diagnosis of epithelial lacrimal gland tumor.