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[An experimental study on compound anisodine III for softening scar of mouse skin after burn]
1Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing.
Objective:
The aim of this study was to investigate the mechanism of compound anisodine (CA) III which can rapidly soften the cicatrized tissue in clinical practice.
Methods:
2 mol/L NaOH was applied onto the back of 36 mice for one minute to induce deep grade skin burn injury. After 1 week local cicatrization occurred. The mice were randomly divided into 3 groups: 1 ml saline was used in group I as control, hyaluronidase 150 U dissolved in 1 ml 2% procaine was used in group II and 0.005% CA 0.1 ml in 2% procaine 1 ml with hyaluronidase 150 U was used in group III. Subcutaneous injection once every other day for two weeks was the method of drug administration in all the 3 groups and then all the rats were euthanized. The burned skin was sectioned for HE and modified Masson's stains.
Results:
Histopathology revealed there were epidermis proliferation, keratinization and fibrosis with disorderly arranged collagenous fibers invading into muscles and a large amount of inflammatory cells infiltrating the lesion in groups I and II. However, the skin was nearly normal in group III.
Conclusions:
CA III can inhibit the epidermic and collagenous proliferation, improve the tissue blood supply and have antiinflammatory effect to prevent scar formation and promote epidermic repair in the skin.