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[Resistance of Haemophilus sp. to antibiotics]
G Janicka1, E Gospodarek, A Mikucka
1Katedry i Zakładu Mikrobiologii Państwowego Szpitala Klinicznego Akademii Medycznej w Bydgoszczy.
Abstract:
The present study was undertaken to determine the in vitro drug resistance of Haemophilus influenzae (68 isolates) and H. parainfluenzae (17 isolates). The tests susceptibility to Ampicillin, Amoxicilin/Clavulanic Acid, Cefaclor, Cefuroxime, Cotrimoxazole, Aztreonam, Ceftriaxone, Tetracycline, Ciprofloxacin, Rifampicin and Chloramphenicol were performed with a standard disk-diffusion method. The NCCLS methodology and susceptibility interpretative criteria were applied as described by the disk manufacturer. Beta-lactamase production was detected with nitrocefin impregnated disk (Cefinase, BBL Microbiology System). Resistance in nosocomially acquired Haemophilus isolates to several antibiotics was observed. Of the Haemophilus isolates 28.2% were Ampicillin in resistant, all were susceptible to the combination of Amoxicillin/Clavulanic acid. The Ampicillin-resistant strains were beta-lactamase producers. We observed the high resistance (70.1%) to Tetracycline and (28.2%) to SXT (Cotrimoxazole). All isolates of Haemophilus were susceptible to Ciprofloxacin. The low resistance percentages to Rifampin (1.2%), Aztreonam (3.5%) and Chloramphenicol (3.5%) was observed.
Insights
This study assessed antibiotic resistance in Haemophilus influenzae and H. parainfluenzae. Most isolates were susceptible to Ciprofloxacin, while high resistance to Ampicillin and Tetracycline was noted.
Area of Science:
- Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Context:
- Haemophilus influenzae and H. parainfluenzae are significant human pathogens.
- Nosocomial infections pose a growing challenge due to antibiotic resistance.
- Understanding antimicrobial susceptibility patterns is crucial for effective treatment.
Purpose:
- To determine the in vitro drug resistance of Haemophilus influenzae and H. parainfluenzae isolates.
- To evaluate susceptibility to a panel of 11 antibiotics using standard disk-diffusion methods.
- To identify beta-lactamase production in resistant strains.
Summary:
- A total of 85 Haemophilus isolates (68 H. influenzae, 17 H. parainfluenzae) were tested against Ampicillin, Amoxicillin/Clavulanic Acid, Cefaclor, Cefuroxime, Cotrimoxazole, Aztreonam, Ceftriaxone, Tetracycline, Ciprofloxacin, Rifampicin, and Chloramphenicol.
- 28.2% of isolates were resistant to Ampicillin, and these strains produced beta-lactamase. All isolates were susceptible to Amoxicillin/Clavulanic Acid.
- High resistance rates were observed for Tetracycline (70.1%) and Cotrimoxazole (28.2%). All isolates remained susceptible to Ciprofloxacin. Low resistance was noted for Rifampin (1.2%), Aztreonam (3.5%), and Chloramphenicol (3.5%).
Impact:
- Findings highlight the emergence of antibiotic resistance in Haemophilus species, particularly to Ampicillin and Tetracycline.
- The effectiveness of Amoxicillin/Clavulanic Acid and Ciprofloxacin as treatment options is reinforced.
- Data provides valuable insights for guiding antimicrobial therapy and infection control strategies against these pathogens.