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Phenol extraction of 28 S ribosomal RNA from rat liver cytoplasm
Preparative Biochemistry
|January 1, 1976
Abstract:
A simple and reproducible phenol method for the isolation of 28 S ribosomal RNA from rat liver cytoplasm, free from poly(A)+/-RNA is described. The procedure is based on the observation that at lower pH of the homogenate (pH 5.5) 28 S ribosomal RNA is extracted, while 18 S ribosomal RNA remains in the interphase layer.