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Pregnancies from subzonal insemination of unfertilized oocytes after conventional IVF
E P Loong1, T T Chiu, C J Haines
1Department of Obstetrics and Gynaecology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.
Objective:
To evaluate the efficacy of subzonal insemination (SUZI) as applied to unfertilized oocytes after initial insemination.
Methods:
Unfertilized oocytes at metaphase II obtained after conventional IVF from 14 patients were selected for reinsemination by SUZI. The fertilization rate and pregnancy outcome were assessed.
Results:
Of the 57 unfertilized oocytes at metaphase II, 10 (17.5%) became fertilized after SUZI. Eight of the 10 (80%) oocytes showed normal fertilization with two pronuclei while the other two (20%) oocytes were polyspermic. Five (35.7%) of the 14 patients had embryo transfer resulting in two (14.3%) pregnancies. One patient had a spontaneous abortion and the other had a live birth at term.
Conclusion:
Reinsemination of unfertilized oocytes by SUZI could be of value when fertilization fails after initial insemination.