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Updated: Aug 12, 2026
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
Mucosa cell regeneration following intestinal ischemia/reperfusion injury in rats
1Department of Pediatric Surgery, Children's Hospital, Shanghai Medical University, China.
Objective:
To evaluate regeneration and proliferation of the mucosa cells following intestinal ischemia/reperfusion (I/R) injury.
Methods:
Sixty rats (200-250 g) were examined after intestinal I/R injury with immunohistochemical (BrdU) method and flow cytometric analysis.
Results:
Severe damage to the villi was evident at 2 hours reperfusion and the rate of cell proliferation was lower (P < 0.01). All the specimens has healed mucosa with re-epithelialized villi tips after 2 days regeneration, but the villi were scarce and tiny. After two and four days regeneration, the rate of cell proliferation was significantly higher than that of controls (P < 0.01).
Conclusions:
Our study suggests that the intestinal regeneration following I/R injury in rats occurs early. The immunohistochemical method with BrdU-labeled technique is a rapid method for quantifying the rate of cellular DNA synthesis.
