Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Simple method for determination of hematopoietic stem cells]

T Yamane1, K Takekawa, M Hino

  • 1Department of Clinical Hematology, Osaka City University Medical School.

Rinsho Byori. the Japanese Journal of Clinical Pathology
|May 22, 1998
PubMed
Summary

A new method using the immature information channel (IMI) on the SE-9000 hematology analyzer rapidly determines peripheral blood stem cells (PBSC). This technique aids in monitoring PBSC levels after chemotherapy, offering clinical utility for patient recovery.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fecal Bacteria Contamination of Floodwaters and a Coastal Waterway From Tidally-Driven Stormwater Network Inundation.

GeoHealth·2024
Same author

Acquired angioedema as a late-onset complication after cord blood transplantation: a subtype of chronic graft-versus-host disease.

QJM : monthly journal of the Association of Physicians·2024
Same author

Adjuvant everolimus in high-risk diffuse large B-cell lymphoma: final results from the PILLAR-2 randomized phase III trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2017
Same author

Pulsed neutron time-dependent intensity modulation for quasi-elastic neutron scattering spectroscopy.

The Review of scientific instruments·2016
Same author

Observation on the Visibility Decrease in a VCN Spin Resonator Interferometry.

Journal of research of the National Institute of Standards and Technology·2016
Same author

Investigation of Solid D 2 for UCN Sources.

Journal of research of the National Institute of Standards and Technology·2016

Area of Science:

  • Hematology
  • Cell Biology
  • Medical Technology

Background:

  • Accurate quantification of peripheral blood stem cells (PBSC) is crucial for monitoring patient recovery post-chemotherapy.
  • Current methods for PBSC determination can be time-consuming and complex.
  • Automated hematology analyzers offer potential for rapid cell counting.

Observation:

  • The immature information channel (IMI) of the SE-9000 analyzer provides data on immature leukocytes.
  • CD34-positive cells, indicative of hematopoietic stem cells, were identified within specific low Recurrent Frequency gates on the IMI channel analysis.
  • A dedicated software program (HPC) was developed to specifically detect cells within this identified gate.

Findings:

  • A strong regression coefficient (0.81) was observed between the ratio of IMI-positive cells and CD34-positive cells in peripheral blood samples from patients before and after chemotherapy (n=122).

Related Experiment Videos

  • The SE-9000 analyzer, utilizing the IMI channel and HPC program, enables rapid and easy determination of PBSC counts.
  • IMI positive cell measurement correlates well with CD34 positive cell counts.

    • Implications:

    • The IMI channel analysis on the SE-9000 provides a clinically useful, rapid, and easy method for monitoring PBSC mobilization after chemotherapy.
    • This approach can streamline the assessment of stem cell recovery in cancer patients.
    • Potential for improved patient management and treatment efficacy through timely monitoring of stem cell levels.