Modification of postradiative changes of nucleic acids by the bacterial extract broncho-vaxom in rat tissues

We have studied the influence of immunomodulator Broncho-Vaxom on the concentration and the total content of RNA and DNA in the bone marrow, spleen and regenerating liver (after partial hepatectomy) in rats irradiated with doses of 3 Gy, 6 Gy or 9 Gy of gamma radiation. Animals in all groups were partially hepatectomized (2/3 of liver mass removed) within 30 min or on the 6th and 13th day after irradiation and they were examined 30 h after resection, i.e., on the 1st, 7th and 14th day after irradiation. Broncho-Vaxom was administered intraperitoneally at the dose of 1.5 mg/rat 24 h before irradiation. We have found that administration of Broncho-Vaxom decreased the loss of RNA and DNA induced by radiation in the bone marrow and partly also in the spleen of rats irradiated with doses 3 and 6 Gy over the whole examined period with a maximum on the 14th day after irradiation. The effect of Broncho-Vaxom was less noticeable in the regenerating liver and appeared especially on the 7th day after irradiation.