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Regulation of distal nephron K+ channels (ROMK) mRNA expression by aldosterone in rat kidney
A H Beesley1, D Hornby, S J White
1Laboratory for Membrane Protein Function, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Abstract:
1. The expression of ROMK mRNA isoforms in rat kidney was measured using competitive polymerase chain reaction. Under basal conditions the expression of ROMK2 and 3 mRNA was significantly higher than that of ROMK1 or 6. 2. Administration of aldosterone for a period of 1 week significantly increased the mRNA expression not only of the alpha1-subunit of the Na+-K+-ATPase, but also of ROMK2, 3 and 6. 3. These data not only provide evidence that ROMK K+ channels may be involved with mineralocorticoid-sensitive K+ secretion in the distal nephron, but also demonstrate for the first time that ROMK6 may be involved in this process.