Related Experiment Video
Updated: Aug 15, 2026
The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
Published on: August 11, 2008
[Detection of M. tuberculosis streptomycin-resistant gene]
Objective:
To observe the mutations of rpsL gene in M. tuberculosis streptomycin-resistant isolates, and to develop a new method for detecting drug resistance.
Method:
Detecting the rpsL genes with PCR-SSCP and analyzing their codons 43 by PCR-RFLP.
Results:
Strain H37Rv was used as a control. In 22 M. tuberculosis clinical isolates, the rpsL PCR fragments from 5 drug-susceptible isolates had no differences in the SSCP profiles with strain H37Rv, and were restricted by Mbo II. 11 of the 13 streptomycin-resistant isolates showed apparent differences in the SSCP profiles and were not digested with Mbo II. 1 of the 4 other drug-resistant isolates also had apparent SSCP differences and was not digested by Mbo II.
Conclusions:
The results suggested that the rpsL gene mutation was frequently observed in M. tuberculosis streptomycin-resistant isolates, and usually situated at codon 43, PCR-SSCP and PCR-RFLP might become a simple, rapid and reliable diagnostic test for drug resistance.
Related Concept Videos
01:26Pulmonary Tuberculosis IV
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
01:28Pulmonary Tuberculosis V
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
01:25Mechanism of Antibiotic Resistance in MRSA