Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of small plasmids from Staphylococcus aureus

S Löfdahl, J E Sjöström, L Philipson

    Gene
    |April 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The gene for staphylococcal protein A.

    Immunology today·2014
    Same author

    Single cell assay with an automated capillary microinjection system.

    Cytotechnology·2012
    Same author

    Purification of B. megatherium Phage G and Evidence for a Muralytic Enzyme as an Integral Part of the Phage.

    The Journal of general physiology·2009
    Same author

    An outbreak of Salmonella Typhimurium infections in Denmark, Norway and Sweden, 2008.

    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2009
    Same author

    A large Escherichia coli O157 outbreak in Sweden associated with locally produced lettuce.

    Foodborne pathogens and disease·2008
    Same author

    The coxsackievirus and adenovirus receptor.

    Current topics in microbiology and immunology·2008

    Researchers characterized Staphylococcus aureus plasmids, finding that pS194 and pC194 exhibit high transforming efficiency. A recombinant plasmid, pSC194, also shows efficient transformation and potential as a vector for hybrid DNA construction.

    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • Plasmids are crucial genetic elements in bacteria, often conferring advantageous traits like antibiotic resistance.
    • Understanding plasmid characteristics is vital for genetic manipulation and understanding bacterial evolution.

    Purpose of the Study:

    • To characterize small molecular weight plasmids from Staphylococcus aureus.
    • To determine their size, restriction enzyme cleavage patterns, and transforming capacity.
    • To evaluate a natural recombinant plasmid for potential use as a DNA vector.

    Main Methods:

    • Zonal rate centrifugation and electron microscopy for DNA size determination.
    • Restriction enzyme digestion (HindIII, EcoRI) to analyze cleavage patterns.
    • Transformation assays in Staphylococcus aureus and Bacillus subtilis.

    Related Experiment Videos

    Main Results:

    • Plasmids pS194 (3.0 Mdal, streptomycin resistance) and pC194 (2.0 Mdal, chloramphenicol resistance) demonstrated high transforming efficiency in S. aureus.
    • pC194 had one HindIII site; pS194 had one HindIII and one EcoRI site.
    • The recombinant plasmid pSC194 possessed high transforming capacity and was efficiently transformed in both S. aureus and B. subtilis.

    Conclusions:

    • The characterized plasmids, pS194 and pC194, are suitable for genetic studies in S. aureus.
    • The recombinant plasmid pSC194 is a promising vector for constructing and propagating hybrid DNA in S. aureus.
    • pSC194's ability to transform B. subtilis suggests broader applicability in molecular biology.