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Freeze-dried human leukocytes stabilized with uranyl acetate during low temperature embedding or with OsO4 vapor
1Universität des Saarlandes, Homburg-Saar, Germany.
Scanning Microscopy. Supplement
|January 1, 1996
Abstract:
Two new simple stabilization procedures for freeze-dried biological material are introduced which are compatible with low temperature embedding (LTE) in Lowicryl. The first method uses a Lowicryl K11M/HM20 mixture supplemented with 0.3% uranyl acetate for LTE. For the second method polymerized Lowicryl blocks containing the freeze-dried material are exposed to OsO4 vapor which penetrates into the Lowicryl block and stabilizes the embedded specimen. The quality of structural preservation is demonstrated with human leukocytes.