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Related Experiment Videos

Development-dependent expression of complex-type sugar chains specific to mouse brain

S Nakakita1, S Natsuka, K Ikenaka

  • 1Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560, Japan.

Journal of Biochemistry
|May 30, 1998
PubMed
Summary
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Two brain-specific sugar chains, BA-1 and BA-2, show distinct developmental expression patterns in mouse cerebrum and cerebellum. These complex carbohydrates are linked to membrane-associated glycoproteins, indicating their role in subcellular organelle function.

Area of Science:

  • Neuroscience
  • Glycobiology
  • Developmental Biology

Background:

  • Previously identified fucosylagalactobiantenna structures (BA-1 and BA-2) are enriched in mouse brain.
  • BA-1 lacks a GlcNAc residue, while BA-2 possesses a bisecting GlcNAc residue.

Purpose of the Study:

  • To quantify the developmental expression of BA-1 and BA-2 in different regions of the mouse brain.
  • To investigate the association of BA-1 and BA-2 with specific glycoprotein fractions.

Main Methods:

  • Pyridylamino sugar chains were prepared from mouse brains of various ages.
  • High-performance liquid chromatography (HPLC) was used to separate and quantify BA-1 and BA-2.
  • Lectin-affinity chromatography and gel filtration were employed to purify associated glycoproteins.

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Main Results:

  • In cerebrum, BA-1 expression increased with development, while BA-2 peaked early and then decreased.
  • Cerebellar BA-1 expression remained lower than BA-2 throughout development.
  • BA-1 and BA-2 were found in glycoproteins >20 kDa in cerebellum and 80-200 kDa in cerebrum, enriched in the membrane fraction.

Conclusions:

  • BA-1 and BA-2 exhibit distinct, developmentally regulated expression patterns in the mouse brain.
  • These brain-specific sugar chains are associated with membrane-bound glycoproteins in subcellular organelles.