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[Physico-chemical properties of there phages of Pseudomonas syringae]
A L Boĭko1, L I Semchuk, L V Tokarchuk
1Taras Shevchenko National University, Kyiv, Ukraine.
Abstract:
The properties of DNA for 9B, 123, 788/8 phages lysing phytopathogenic Pseudomonas syringae bacteria have been analysed with results presented. It was ascertained that their genomes consist of GC-type two-chain DNA molecules having molecular weight 14-15 mDa. It was shown that sedimentation coefficient for all three phage DNA is identical and equals 26S. GC-base percentage was calculated for the phage genomes. Its value, according to results of sedimentation analysis and melting temperature, was the same for 9B (51%, 57%) and 123 (51%, 57%) and differed for 788/8 (53%, 60%). The molecular weight of DNA phages calculated from the sum of fragments, obtained after genome splitting with restriction endonucleases is in agreement with the data of sedimentation analysis. The distribution of phage DNA fragments if electrophoregrams suggests the presence of numerous common restriction sites in the phages' genomes.
Insights
This study analyzed DNA from three Pseudomonas syringae phages, finding they possess similar GC-type DNA. Their genomes share common restriction sites, indicating potential genetic relatedness.
Area of Science:
- Microbiology
- Molecular Biology
- Virology
Background:
- Bacteriophages are viruses that infect bacteria and play crucial roles in microbial ecosystems.
- Pseudomonas syringae is a significant phytopathogenic bacterium affecting various plant species.
- Understanding phage-host interactions is vital for agricultural applications and microbial control.
Purpose of the Study:
- To characterize the DNA properties of three bacteriophages (9B, 123, 788/8) that infect Pseudomonas syringae.
- To determine the molecular weight, sedimentation coefficient, and GC-base percentage of the phage DNA.
- To investigate the genetic relatedness of these phages through restriction endonuclease analysis.
Main Methods:
- Sedimentation analysis to determine DNA sedimentation coefficients.
- Melting temperature analysis to calculate GC-base percentage.
- Restriction endonuclease digestion and subsequent electrophoresis to analyze DNA fragments.
Main Results:
- All three phages possess double-stranded, GC-type DNA with molecular weights between 14-15 mDa.
- Identical sedimentation coefficients (26S) were observed for all three phage DNAs.
- GC-base percentages were 51-57% for phages 9B and 123, and 53-60% for phage 788/8.
- Restriction analysis revealed numerous common restriction sites, suggesting genomic similarities.
Conclusions:
- The analyzed Pseudomonas syringae phages share fundamental DNA characteristics, including molecular weight and GC content.
- The presence of common restriction sites indicates a degree of genomic homology among these phages.
- These findings contribute to the understanding of phage diversity and evolution within the Pseudomonas syringae species.