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Updated: Jul 17, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Analysis of DNA fragments from conventional and microfabricated PCR devices using delayed extraction MALDI-TOF mass
P L Ross1, P A Davis, P Belgrader
1DNA Technology Development Branch, Center for Medical and Molecular Genetics, Armed Forces Institute of Pathology, Rockville, Maryland 20850, USA. philip_ross@pbio.com
Abstract:
Applications of polymerase chain reaction (PCR) product analysis using rapid affinity capture followed by delayed extraction (DE) MALDI-TOFMS is presented. Such applications include multiplex short tandem repeat (STR) typing, which is demonstrated for STR systems from conventional and microchip-based thermal cycling instruments. Using the combination of the microfabricated PCR instrument and DE-MALDI-TOFMS, a complete genotyping assay can be performed in under 50 min with a resultant molecular weight accuracy approaching or exceeding 100 ppm through external calibration. The observed resolution and mass accuracy for a 69-base PCR product enables identification of single base substitutions by direct molecular weight determination.
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