Related Experiment Videos
Integration host factor positively regulates cycJIH transcription
A Sirko1, A Wegleńska, D Hulanicka
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw. asirko@ibbrain.ibb.waw.pl
Molecular & General Genetics : MGG
|June 5, 1998
Abstract:
We report the identification of integration host factor (IHF) as an additional element involved in the regulation of the cysJIH promoter of Salmonella typhimurium and Escherichia coli. An IHF-binding site was located in the regulatory region of the cysJIH promoter by using two methods, protection against DNase I digestion and hydroxyl radical cleavage. The positive influence of IHF on in vitro run-off transcription from the cysJIH promoter is shown. The in vivo observations suggest that IHF is necessary for full expression of cysJIH in stationary phase but not during exponential growth.