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Chromosomal mapping of Tmp (Emp1), Xmp (Emp2), and Ymp (Emp3), genes encoding membrane proteins related to Pmp22
I Ben-Porath1, C A Kozak, N Benvenisty
1Department of Genetics, Hebrew University, Jerusalem, Israel.
Abstract:
We have recently characterized a novel mammalian gene family, encoding membrane glycoproteins with four trans-membrane domains. This gene family includes the previously studied PMP22, which is involved in the Charcot-Marie-Tooth neuropathy, and three novel genes: TMP, XMP, and YMP (HGMW-approved symbols EMP1, EMP2 and EMP3, respectively). The Tmp (tumor-associated membrane protein) gene was isolated from a c-myc induced mouse brain tumor and is expressed in several highly proliferative cell types. We have now isolated cDNAs of the mouse Xmp and Ymp genes and determined the chromosomal localization of mouse Tmp, Xmp, and Ymp. Tmp was mapped to mouse chromosome 6, Xmp was mapped to chromosome 16, and Ymp was mapped to chromosome 7. Tmp and Ymp map to paralogous chromosomal regions, whereas Xmp maps to a chromosomal region that is putatively paralogous to a region on chromosome 11, to which Pmp22 was previously mapped. These data suggest that this family of membrane glycoproteins evolved as a result of chromosomal duplications.
Insights
We identified a new family of membrane glycoproteins, including PMP22 involved in Charcot-Marie-Tooth neuropathy, and three novel genes (TMP, XMP, YMP). Their chromosomal mapping suggests evolution through gene duplication events.
Area of Science:
- Genetics
- Molecular Biology
- Neuroscience
Background:
- A novel mammalian gene family encoding membrane glycoproteins with four trans-membrane domains has been characterized.
- This family includes the known PMP22 gene, implicated in Charcot-Marie-Tooth neuropathy, and three newly identified genes: TMP, XMP, and YMP.
Purpose of the Study:
- To isolate cDNAs for the mouse Xmp and Ymp genes.
- To determine the chromosomal localization of mouse Tmp, Xmp, and Ymp genes.
- To investigate the evolutionary origins of this membrane glycoprotein gene family.
Main Methods:
- Gene isolation and cDNA sequencing for Xmp and Ymp.
- Chromosomal mapping of Tmp, Xmp, and Ymp in mice.
- Comparative genomic analysis to identify paralogous regions.
Main Results:
- The Tmp gene was mapped to mouse chromosome 6, Xmp to chromosome 16, and Ymp to chromosome 7.
- Tmp and Ymp map to paralogous chromosomal regions.
- Xmp maps to a region putatively paralogous to the Pmp22 locus on chromosome 11.
Conclusions:
- The identified membrane glycoprotein gene family likely evolved through chromosomal duplication events.
- The chromosomal mapping provides insights into the evolutionary history and potential functional relationships within the gene family.