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Related Experiment Videos

In vitro organogenesis of liver tissue

L G Griffith1, B Wu, M J Cima

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA. griff@mit.edu

Annals of the New York Academy of Sciences
|June 9, 1998
PubMed
Summary

Researchers are creating vascularized liver tissue outside the body for transplantation. This approach uses advanced polymer scaffolds to organize cells, overcoming limitations of cell mass for patients needing liver tissue.

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Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Hepatology

Background:

  • Hepatocytes have high metabolic rates, limiting transplantable cell mass without vascularization.
  • Current liver transplantation methods face donor shortages and immune rejection challenges.
  • Developing ex vivo vascularized liver tissue offers a potential alternative for liver regeneration.

Purpose of the Study:

  • To address the limitations of cell mass in liver transplantation by developing vascularized hepatic tissue ex vivo.
  • To explore in vitro organogenesis of vascularized liver tissue for potential anastomosis into the portal vein.
  • To present a novel fabrication approach for creating complex, degradable polymer scaffolds for tissue engineering.

Main Methods:

  • Fabrication of complex, degradable polymer scaffolds with micron-scale features.

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  • Three-dimensional cell organization within the fabricated scaffolds.
  • In vitro tissue organization using mixed-cell cultures, including hepatocytes.
  • Assessment of scaffold feasibility for supporting tissue development.

    • Main Results:

    • Demonstrated a fabrication approach for complex, degradable polymer scaffolds.
    • Successfully organized cells in three dimensions on a micron scale using these scaffolds.
    • Showcased the feasibility of using scaffolds for in vitro tissue organization in mixed-cell cultures.
    • Provided insights into key design considerations for in vitro organogenesis of vascularized hepatic tissue.

    Conclusions:

    • The developed polymer scaffolds show feasibility for in vitro hepatic tissue organization.
    • This approach offers a potential solution for overcoming cell mass limitations in liver transplantation.
    • Further development of ex vivo vascularized hepatic tissue holds promise for regenerative medicine applications.