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Updated: Jul 31, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide
P M Desmarchelier1, S S Bilge, N Fegan
1Commonwealth Scientific and Industrial Research Organisation, Brisbane, Australia. Patricia.Desmarchelier@foodscience.afisc.csiro.au
Abstract:
A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected < 1 CFU of E. coli O157 per ml in raw milk following enrichment.
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