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Related Experiment Videos

Gene trap insertion into a novel gene expressed during mouse limb development

A Pires-DaSilva1, P Gruss

  • 1Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Göttingen, Germany.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|June 17, 1998
PubMed
Summary

Gene trapping identified a novel gene, paddy, crucial for embryonic development. Its spatiotemporal expression in limb buds suggests roles in anteroposterior regionalization and posterior determination.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Gene trapping is a valuable technique for discovering novel genes involved in embryonic development.
  • Understanding gene expression patterns is key to elucidating gene function during development.

Purpose of the Study:

  • To characterize the spatiotemporal expression of a newly identified gene, paddy, using a gene-trap approach.
  • To investigate the potential roles of paddy in embryonic development, particularly limb development.

Main Methods:

  • Utilized gene trapping to identify and isolate a novel gene.
  • Employed 5'-rapid amplification of cDNA ends (RACE) to clone a partial cDNA of the endogenous gene.
  • Analyzed the spatiotemporal expression pattern of the gene throughout embryonic development.

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Main Results:

  • Identified and cloned a novel gene, named paddy, with no known sequence similarity.
  • Paddy is first expressed at embryonic day 9.5 (E9.5) in forelimbs and branchial arches.
  • Expression is detected in the stomach, genital bud, and pharyngeal epithelium at E11.5, later appearing in hair follicles, while stomach and pharyngeal expression diminishes.
  • Early paddy expression correlates with anteroposterior (A/P) regionalization in limb buds.
  • Paddy expression fades distally during limb bud elongation, independent of apical ectodermal ridge (AER) and zone of polarizing activity (ZPA) signaling.

Conclusions:

  • The novel gene paddy exhibits dynamic spatiotemporal expression during embryogenesis.
  • Paddy likely plays significant roles in limb development, including anteroposterior regionalization and posterior determination in proximal regions.
  • The distinct expression pattern suggests paddy functions independently of established AER and ZPA signaling pathways in later limb development.