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Successful pregnancy with sperm retrieved from the bladder after the introduction of a low-electrolyte solution for
K Saito1, Y Kinoshita, Y Yumura
1Department of Urology, Yokohama City University School of Medicine, Yokohama, Kanagawa, Japan. kazsaito@yellow.med.yokohama-cu.ac.jp
Objective:
To determine the safety and efficacy of the introduction of a low-electrolyte solution into the bladder before masturbation for sperm recovery in patients with retrograde ejaculation.
Design:
Two case reports.
Setting:
Assisted reproduction practice at a university hospital.
Patient(S):
Two men with retrograde ejaculation.
Intervention(S):
A 0.32-mol/L glucose solution or Ham's modified F-10 medium (GIBCO, Grand Island, NY) with 10% serum was introduced into the urinary bladder before masturbation. The retrieved sperm were used for IUI.
Main Outcome Measure(S):
The motility of retrieved sperm, achievement of pregnancy, and outcome of pregnancy.
Result(S):
In patient 1, the motility rate of sperm retrieved from the bladder after the introduction of Ham's modified F-10 medium was higher than that after the introduction of a glucose solution. The patient's wife did not become pregnant during 10 IUI cycles after treatment with Ham's modified F-10 medium. However, she became pregnant during the first IUI cycle after treatment with a glucose solution and was delivered of healthy twin infants. The wife of patient 2 also became pregnant during the first IUI cycle after treatment with a glucose solution and was delivered of a healthy female infant.
Conclusion(S):
The introduction of a glucose solution appears to be a safe and simple method for sperm recovery in patients with retrograde ejaculation.