Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

beta-Cyclodextrin production by simultaneous fermentation and cyclization

H O Lima1, F F De Moraes, G M Zanin

  • 1State University of Maringá, Chemical Engineering Department, Brazil.

Applied Biochemistry and Biotechnology
|June 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Production and purification of CGTase of alkalophylic Bacillus isolated from Brazilian soil.

Applied biochemistry and biotechnology·2008
Same author

Study of Ca-ATMP precipitation in the presence of magnesium ion.

Langmuir : the ACS journal of surfaces and colloids·2005
Same author

Scale inhibition study by turbidity measurement.

Journal of colloid and interface science·2005
Same author

Bleaching of kraft pulp with commercial xylanases.

Applied biochemistry and biotechnology·2004
Same author

Characterization and utilization of Candida rugosa lipase immobilized on controlled pore silica.

Applied biochemistry and biotechnology·2004
Same author

Modeling cellobiose hydrolysis with integrated kinetic models.

Applied biochemistry and biotechnology·2004
Same journal

Bioactive Compound-Integrated Metal Nanoparticles Promote Wound Repair in Diabetic Conditions: Evidence from an Animal Study.

Applied biochemistry and biotechnology·2026
Same journal

DianDao San Promotes CSU Wound Healing by Inhibiting TNF/NF-κB Pathway.

Applied biochemistry and biotechnology·2026
Same journal

Deciphering Melatonin-Driven Bioenergy Reprogramming Through Integrated Physiological and Metabolomic Analyses in Dunaliella salina.

Applied biochemistry and biotechnology·2026
Same journal

Activated Carbon Functionalization for Lipase Immobilization: Characterization, Hydrolytic Activity, and Ethyl Lactate Synthesis.

Applied biochemistry and biotechnology·2026
Same journal

A Cold- and Salt-Tolerant GH43-1 β-Xylosidase from Cellulomonas bogoriensis 69B4<sup>T</sup>: Biochemical Characterization and Subfamily Functional Diversity.

Applied biochemistry and biotechnology·2026
Same journal

Microbially-Induced Carbonate Precipitation by Carbonic Anhydrases: A Novel Approach for Lead-Zinc Tailings Remediation.

Applied biochemistry and biotechnology·2026
See all related articles

Simultaneous fermentation and cyclization (SFC) enhances beta-cyclodextrin (CD) production from cassava starch. This novel method improves yields by removing inhibitory sugars and producing ethanol, making it less sensitive to starch dextrose equivalent (DE) variations.

Area of Science:

  • Biotechnology
  • Biochemistry
  • Enzymology

Background:

  • Beta-cyclodextrin (CD) production is often inhibited by glucose and maltose.
  • Enzymatic cyclization using CGTase is a primary method for CD synthesis.
  • Optimizing reaction conditions, such as dextrose equivalent (DE) of starch hydrolysates, is crucial for maximizing CD yields.

Purpose of the Study:

  • To investigate a novel simultaneous fermentation and cyclization (SFC) process for enhanced beta-cyclodextrin (CD) production.
  • To compare the yield and DE sensitivity of SFC with traditional CGTase-only and ethanol-supplemented methods.
  • To evaluate the impact of yeast co-incubation on beta-CD production efficiency.

Main Methods:

  • Cassava starch was liquefied with alpha-amylase to achieve varying dextrose equivalents (DE).

Related Experiment Videos

  • Three reaction media were tested: CGTase enzyme only, CGTase with added ethanol (1-5%), and SFC with CGTase, Saccharomyces cerevisiae yeast (12% w/v), and nutrients.
  • Reactions were conducted at 38°C, pH 6.0, with 3.3 mL CGTase/L, across a DE range of 2-25.

    • Main Results:

    • The SFC process yielded significantly higher beta-CD concentrations compared to CGTase-only or ethanol-supplemented methods.
    • Beta-CD yield decreased with increasing DE in all tested conditions.
    • The SFC process demonstrated reduced sensitivity to DE variations, maintaining higher yields across a broader DE range compared to other methods.

    Conclusions:

    • Simultaneous fermentation and cyclization (SFC) is a superior method for producing beta-cyclodextrin (CD) with high yields from cassava starch hydrolysates.
    • The SFC process effectively mitigates the inhibitory effects of glucose and maltose while leveraging ethanol production for yield enhancement.
    • SFC offers a more robust and less DE-sensitive approach to beta-CD production, making it advantageous for industrial applications.