Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA demethylation: turning genes on

Y Bergman1, R Mostoslavsky

  • 1The Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.

Biological Chemistry
|June 17, 1998
PubMed
Summary

DNA methylation regulates eukaryotic gene expression by modulating protein-DNA interactions, primarily silencing transcription. DNA demethylation is an active process crucial for gene regulation, particularly in the immune system.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting mitochondrial translation by inhibiting DDX3: a novel radiosensitization strategy for cancer treatment.

Oncogene·2017
Same author

MicroRNAs in metabolism and metabolic diseases.

Cold Spring Harbor symposia on quantitative biology·2011
Same author

SIRT6 in DNA repair, metabolism and ageing.

Journal of internal medicine·2008
Same author

Asynchronous replication and allelic exclusion in the immune system.

Nature·2001
Same author

Developmental regulation of DNA replication timing at the human beta globin locus.

The EMBO journal·2001
Same author

Demethylation and the establishment of kappa allelic exclusion.

Cold Spring Harbor symposia on quantitative biology·2001

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Genetics

Background:

  • Eukaryotic gene expression involves complex interactions between transacting factors and cis-regulatory elements.
  • DNA methylation acts in cis to influence protein-DNA interactions and modulate gene expression.
  • Methylation is primarily linked to transcriptional silencing via repressive complexes.

Purpose of the Study:

  • To elucidate the role of DNA methylation in eukaryotic gene expression.
  • To understand the mechanisms of DNA demethylation.
  • To highlight the significance of DNA methylation in the immune system.

Main Methods:

  • Review of existing evidence on DNA methylation and gene expression.
  • Analysis of mechanisms underlying transcriptional silencing by methylation.
  • Examination of the enzymatic processes involved in DNA demethylation.

Main Results:

  • DNA methylation modulates protein-DNA interactions, affecting gene expression.
  • Transcriptional silencing is often achieved indirectly through methylation-induced complex assembly.
  • DNA demethylation is an active, enzymatically controlled process influenced by cis-regulatory elements.

Conclusions:

  • DNA methylation is a key epigenetic mechanism regulating gene expression.
  • Active DNA demethylation is essential for controlling gene activity.
  • DNA methylation plays critical roles in immune system function, including gene expression and rearrangement.

Related Experiment Videos