Related Experiment Videos
An experimental selection system to identify bacterial cells exhibiting a new DNA host specificity
A Kunz1, A Meisel, P Mackeldanz
1Institute of Medical Virology, Charité Medical School, Humboldt University, Berlin, Germany.
Biological Chemistry
|June 17, 1998
Abstract:
Restriction-modification enzymes interact with DNA sequences in a highly specific manner. Mutations within the DNA binding region of the enzymes could be expected to produce enzyme variants with changed DNA sequence specificities. We developed an efficient in vivo selection system that enabled us to detect one cell coding for a restriction-modification system with a new DNA sequence specificity in a background of more than 10(6) cells with the original DNA sequence specificity.