Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

pH-sensitive, cationic liposomes: a new synthetic virus-like vector

V Budker1, V Gurevich, J E Hagstrom

  • 1Department of Pediatrics, Waisman Center, University of Wisconsin, Madison 53705, USA.

Nature Biotechnology
|June 1, 1996
PubMed
Summary

Researchers developed pH-sensitive liposomes for efficient DNA delivery into cells. These cationic liposomes show promise for gene therapy applications by improving DNA transfer and cellular uptake.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep microbial analysis of multiple placentas shows no evidence for a placental microbiome.

BJOG : an international journal of obstetrics and gynaecology·2019
Same author

Wilm's Tumor: An Update.

Cancer·2018
Same author

Improvement of ELISA procedures through simultaneous addition of antigen and detection antibody and elimination of washing steps.

Journal of immunoassay & immunochemistry·2017
Same author

An improved method for the determination of Fc function of immunoglobulins.

Vox sanguinis·2009
Same author

Nutritional management of PKU with glycomacropeptide from cheese whey.

Journal of inherited metabolic disease·2008
Same author

Propagation of anisotropic Bessel-Gaussian beams: sidelobe control, mode selection, and field depth.

Applied optics·2008

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Gene therapy requires efficient and safe methods for delivering genetic material into target cells.
  • Liposomes are versatile carriers for drug and gene delivery, but their efficiency can be limited.
  • pH-sensitive liposomes offer potential advantages by responding to the acidic environment of endosomes.

Purpose of the Study:

  • To synthesize and characterize novel cationic, pH-sensitive liposomes for efficient DNA transfer.
  • To investigate the mechanism of DNA delivery mediated by these liposomes in various cell types.
  • To evaluate the potential of these liposomes as reagents for gene therapy.

Main Methods:

  • Synthesis of cationic lipids with a pK in the physiological range (4.5-8).

Related Experiment Videos

  • Incorporation of cationic lipids with dioleoylphosphatidylethanolamine into liposomes.
  • Assessment of DNA binding, incorporation, and fusion under acidic conditions.
  • Evaluation of transfection efficiency in cultured cells, correlating with endosomal acidification.

    • Main Results:

    • Cationic, pH-sensitive liposomes efficiently mediated DNA transfer into diverse cultured cells.
    • Acidic conditions were found to promote DNA binding, incorporation, and fusion mediated by the liposomes.
    • Transfection efficiency was directly dependent on endosomal acidification, similar to virus-mediated entry.
    • The liposomes demonstrated effective DNA delivery, suggesting a promising new reagent class.

    Conclusions:

    • Cationic, pH-sensitive liposomes are effective reagents for mediating efficient DNA transfer into cells.
    • The pH-sensitivity of the liposomes is crucial for their function, particularly in endosomal release.
    • These liposomes represent a promising advancement for gene therapy applications due to their efficient transfection capabilities.