[Solid-phase immunoenzyme analysis of chloramphenicol in human blood serum]

A Iu Kolosova¹, Zh V Samsonova, A N Blintsov

¹Department of Chemistry, M.V. Lomonosov Moscow State University.

Voprosy Meditsinskoi Khimii

|June 23, 1998

Abstract:

The method of enzyme linked immunosorbent assay (ELISA) for quantitative detection of chloramphenicol (CAP) in human blood serum was developed. Peculiarities of the adsorption on the microtitre plates surface of CAP-ovalbumin conjugate were investigated. Different conditions of competition stage of the analysis were studied. Conditions providing CAP monitoring in human blood serum in the clinical range were optimized. Matrix effect on the assay results was studied. The specificity of the analytical system was investigated and the reagents stability was examined. The method developed permits CAP concentration to be determined in human blood serum, diluted 1/100, in the linear range from 10 to 1000 ng/ml. The assay is characterized by high sensitivity (1 ng/mL) and good reproducibility (CV < 12%), assay time is about 3 hours.

Related Experiment Videos

[Solid-phase immunoenzyme analysis of chloramphenicol in human blood serum]

Related Concept Videos

Properties and Activity of Peptide Derivatives of ACE2 Cellular Receptor and Their Interaction with SARS-CoV-2 S Protein Receptor-Binding Domain.

Peptide Inhibitors of the Interaction of the SARS-CoV-2 Receptor-Binding Domain with the ACE2 Cell Receptor.

[Peptide inhibitors of the interaction of the SARS-CoV-2 receptor-binding domain with the ACE2 cell receptor].

Inhibitors of β-Lactamases. New Life of β-Lactam Antibiotics.

Overcoming Antibiotic Resistance in Microorganisms: Molecular Mechanisms.

[Scaffold hopping computational approach for searching novel β-lactamase inhibitors].

[Study of functional activity of components and factors of the human complement system].

[Stability of immobilized insulin preparation at various pH].

[Characterization of cysteine proteinase endogenous inhibitors obtained from transformed fibroblasts].

[Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa)].

[Inhibition by IFN-(Asp)4-Lys-HIV chimeric protein of hydrolysis of the low molecular substrate by the enteropeptidase light chain].

[Tripeptidyl peptidase 1 deficiency in neuronal ceroid lipofuscinosis. A novel mutation].

Automatic Detection of Mild Intracranial Stenosis using Time-of-Flight Magnetic Resonance Angiography.

Quantitative Fluorescence Imaging of Alphavirus Infection for Antiviral Screenings.

General, operationally simple N-dealkylation of simple and complex tertiary amines.

High-throughput analysis of emerging contaminants in biosolids, composts and soils using liquid chromatography coupled to high resolution orbitrap mass spectrometry.

Pick Your Own Adventure: Orthogonal Methods for Analyzing Polyphenols.

Low-Dose Chlorothalonil Induces Testicular Injury Through Disruption of Steroidogenic Signaling in Adult Male Mice.

Related Experiment Videos

[Solid-phase immunoenzyme analysis of chloramphenicol in human blood serum]

Abstract:

Related Concept Videos

Related Articles