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Vinculin and cell-cell adhesion

A Tozeren1, S Wu, B Hoxter

  • 1Biomedical Engineering Program, Catholic University of America, Washington, DC 20064, USA.

Cell Adhesion and Communication
|June 25, 1998
PubMed
Summary
This summary is machine-generated.

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Vinculin is not essential for strong cell-cell adhesion in F9 carcinoma cells when they are attached to a surface. However, vinculin loss weakens cell-cell adhesion in aggregates formed in suspension.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Vinculin is a protein found in adhesion plaques and adherence junctions in non-muscle cells.
  • Its precise role in the physical strength of cell-cell adhesion requires further investigation.

Purpose of the Study:

  • To investigate the role of vinculin in the physical strength of cell-cell adhesion.
  • To determine if vinculin is necessary for maintaining cell-cell adhesion in F9 mouse embryonal carcinoma cells.

Main Methods:

  • Disaggregation assays were performed on aggregates of wild-type F9 cells, vinculin-depleted F9 cell lines, and a reconstituted vinculin-expressing cell line.
  • Immunoblotting was used to assess the expression of E-cadherin and catenins.
  • Double immunofluorescence microscopy visualized vinculin localization.

Related Experiment Videos

  • Laminar flow assays measured the strength of cell-cell adhesion under shear stress.

    • Main Results:

    • Vinculin-positive and vinculin-negative cell aggregates formed in culture for 24-48 hours remained intact under high shear rates, indicating substrate-adherent cells form strong, vinculin-independent adhesions.
    • Aggregates of vinculin-depleted cells formed in suspension were more easily disaggregated by shear flow compared to vinculin-positive aggregates.
    • Loss of vinculin reduced cell-cell adhesion strength specifically in cells lacking substrate contact.

    Conclusions:

    • Vinculin is not required for forming strong cell-cell adhesion bonds between neighboring carcinoma cells adherent to the basal lamina.
    • Vinculin plays a significant role in maintaining cell-cell adhesion strength in aggregates formed in suspension (lacking substrate contact).