[Studies on the molecular weight standards for size-exclusion chromatography--standardization of a molecular weight...

The possibility of the standardization of molecular weight standards for size-exclusion chromatography (SEC) was investigated in the case of water-soluble polysaccharides such as dextran, hyaluronate and chitosan. In the case of dextran, comparing the method adopted for dextran in EP and BP, it was shown that pullulan, one of commercially available molecular weight standards, can be used in usual SEC method. For hyaluronate and chitosan, applying the Mark-Houwink correction to the calibration curve obtained by pullulan, the acceptable molecular weights could be estimated.