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Related Experiment Videos

p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13

T Kozasa1, X Jiang, M J Hart

  • 1Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA.

Science (New York, N.Y.)
|June 26, 1998
PubMed
Summary
This summary is machine-generated.

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Regulators of G protein signaling (RGS) proteins enhance GTPase activity. p115 RhoGEF acts as a GTPase activating protein for G12 and G13 alpha subunits, suggesting a role in Rho protein regulation.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Biochemistry

Background:

  • Regulators of G protein signaling (RGS) proteins are known to stimulate the GTPase activity of specific G protein alpha subunits.
  • p115 RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho, possesses an amino-terminal region homologous to RGS proteins.

Purpose of the Study:

  • To investigate the GTPase activating protein (GAP) activity of p115 RhoGEF and its N-terminal domain.
  • To determine the specificity of p115 RhoGEF's GAP activity towards different G protein alpha subunits.

Main Methods:

  • Recombinant expression of p115 RhoGEF and its N-terminal fusion protein.
  • Assays to measure GTPase activating protein activity on various G protein alpha subunits (G12, G13, Gs, Gi, Gq).

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Main Results:

  • Recombinant p115 RhoGEF exhibited specific GTPase activating protein activity towards the alpha subunits of G12 and G13.
  • The N-terminal domain of p115 RhoGEF was sufficient for this specific activity.
  • No significant activity was observed towards Gs, Gi, or Gq alpha subunits.

Conclusions:

  • p115 RhoGEF functions as a GTPase activating protein for G12 and G13 alpha subunits.
  • This suggests a potential role for p115 RhoGEF as an intermediary in the signaling pathways regulated by G12 and G13 proteins.
  • The findings highlight a novel function for a GEF protein within the RGS protein-like domain context.