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[Potentiometric electrodes for determining choline, butyrylcholine and cholinesterase inhibitors]
1All-Russia Research Center for Molecular Diagnosis and Treatment, Moscow, Russia.
Abstract:
Potentiometric choline electrodes were developed on the basis of the mediator-free bioelectrocatalysis. The electrodes made of a composite carbon-polymer material contain choline oxidase and peroxidase coimmobilized on the surface of the electrode. The rate of the potential increase was shown to be proportional to the choline concentration within a broad range of variation. Coupling of choline-sensitive electrodes with butyrylcholinesterase makes possible both the direct detection of butyrylcholine and analysis of butyrylcholinesterase inhibitors.
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