Cytokines in hematopoiesis

Hematopoiesis is the process by which mature, functional progeny of the eight major lineages of blood cells are produced from a hierarchy of progressively less mature progenitor and stem cells. The control of hematopoiesis involves intimate cellular interactions between developing blood cells and stromal elements as well as regulation by soluble cytokines, that may act locally in the bone marrow environment or at remote tissue sites. In excess of twenty cytokines that stimulate the production and/or function of hematopoietic cells have now been cloned and are available in purified, recombinant form. The colony-stimulating factors, erythropoietin and the recently discovered thrombopoietin are key regulators of granulocyte/macrophage, erythroid and megakaryocyte/platelet production respectively. The activities of these cytokines have been extensively studied, both in vitro and in vivo, and recent analysis of mice genetically engineered to lack these regulators or their cell surface receptors have provided profound insights into their essential physiological roles. These studies have culminated in the development of these cytokines as valuable clinical reagents.