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Optimal membrane choice for microdialysis sampling of oligosaccharides
N Torto1, J Bång, S Richardson
1Department of Analytical Chemistry, University of Lund, Sweden.
Journal of Chromatography. A
|July 1, 1998
Summary
A new method using microdialysis and chromatography monitors oligosaccharides in bioprocesses. This technique is stable at high temperatures and shows consistent extraction fractions across various concentrations.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Bioprocess Engineering
Background:
- Monitoring oligosaccharides is crucial for optimizing bioprocesses.
- Existing analytical methods may face challenges with complex biological matrices and varying conditions.
Purpose of the Study:
- To develop and validate a robust analytical methodology for oligosaccharide monitoring in bioprocesses.
- To evaluate the performance of different microdialysis membranes under various conditions.
Main Methods:
- Utilized microdialysis sampling coupled with high-performance anion-exchange chromatography and electrochemical detection.
- Tested membranes (3-100 kDa cut-off) for extraction fraction (EF), permeability, temperature stability, and enzyme interaction.
- Employed maltooligosaccharide standards and amylopectin for method validation.
Main Results:
- The developed method effectively monitored oligosaccharides, including glucose to maltoheptaose.
- Membrane performance varied, with polysulfone showing higher EF and enzyme interaction than polyamide and polyethersulfone.
- Extraction fraction remained concentration-independent across a 250-fold range.
- Membranes demonstrated excellent thermal stability up to 90°C for 24 hours.
Conclusions:
- The presented analytical methodology is versatile and reliable for oligosaccharide monitoring in bioprocesses.
- Polyamide and polyethersulfone membranes are preferable due to lower enzyme interaction.
- The method's high-temperature stability makes it suitable for demanding bioprocess applications.