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Derivatization in capillary electrophoresis

H A Bardelmeijer1, H Lingeman, C de Ruiter

  • 1Utrecht University, Faculty of Pharmacy, Department of Pharmaceutical Analysis, Netherlands.

Journal of Chromatography. A
|July 1, 1998
PubMed
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Capillary electrophoresis (CE) offers simple and efficient separation but suffers from low sensitivity. Derivatization, particularly with fluorescence detection, enhances analyte detection for pharmaceutical, biomedical, and environmental samples.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrophoresis (CE) is a versatile separation technique comparable to gas and liquid chromatography (LC).
  • CE offers simplicity and efficiency but faces limitations in detection sensitivity and selectivity.
  • Derivatization is a key strategy to enhance detection characteristics in CE analysis.

Purpose of the Study:

  • To provide an overview of derivatization reagents for capillary electrophoresis.
  • To discuss the advantages and disadvantages of various derivatization modes (pre-, post-, on-column).
  • To explore fluorescence detection and laser-based methods in conjunction with derivatization for improved CE sensitivity.

Main Methods:

  • Review of derivatization reagents and techniques applicable to CE.

Related Experiment Videos

  • Comparison of derivatization strategies in CE versus LC.
  • Discussion of fluorescence principles and requirements for optimal labeling.

    • Main Results:

    • Derivatization significantly improves sensitivity and selectivity in CE.
    • Fluorescence detection is a favored method when combined with derivatization.
    • Specific emphasis on reagents for labeling amino, aldehyde, keto, carboxyl, hydroxyl, and sulfhydryl groups.

    Conclusions:

    • Derivatization is crucial for overcoming sensitivity limitations in CE.
    • Fluorescence detection, especially with diode lasers, offers high potential for CE analysis.
    • The choice of derivatization reagent depends on the target analyte functional groups.