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BfiI, a restriction endonuclease from Bacillus firmus S8120, which recognizes the novel non-palindromic sequence
J Vitkute1, Z Maneliene, M Petrusyte
1Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.
Nucleic Acids Research
|July 3, 1998
Abstract:
A new type IIS restriction endonuclease Bfi I hasbeen partially purified from Bacillus firmus S8120. Bfi I recognizes the non-palindromic hexanucleotide sequence 5'-ACTGGG(N)5/4-3' and makes a staggered cut at the fifth base pair downstream of the recognition sequence on the upper strand, producing a single base 3' protruding end.