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Related Experiment Videos

Desmosomal adhesion inhibits invasive behavior

C Tselepis1, M Chidgey, A North

  • 1Epithelial Morphogenesis Research Group, School of Biological Sciences, University of Manchester, 3.239 Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|July 8, 1998
PubMed
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Desmosomes, crucial for tissue structure, were engineered into non-adhesive cells. This desmosomal adhesion significantly inhibited cancer cell invasion, suggesting a role in suppressing tumor spread.

Area of Science:

  • Cell Biology
  • Cancer Research
  • Molecular Biology

Background:

  • Desmosomes are vital for tissue architecture and integrity.
  • Down-regulation of desmosomal components is observed in carcinomas, hinting at a role in suppressing tumor invasion and metastasis.
  • Functional evidence for desmosomes' role in inhibiting cancer spread is lacking.

Purpose of the Study:

  • To generate functional desmosomal adhesion in an invasive cell line.
  • To investigate the role of desmosomal components in cell adhesion and invasion.
  • To provide functional evidence for desmosomes in suppressing tumor metastasis.

Main Methods:

  • Transfection of L929 fibroblasts with desmosomal components (desmocollin, desmoglein, plakoglobin).
  • Cell aggregation assays to assess adhesion.

Related Experiment Videos

  • Invasion assays using collagen gels.
  • Treatment with anti-adhesion peptides targeting desmocollin and desmoglein recognition sites.

    • Main Results:

    • Expression of desmosomal components in L929 fibroblasts generated specific cell adhesion.
    • This desmosomal adhesion was blocked by peptides targeting desmocollin and desmoglein.
    • Transfected cells exhibited substantially inhibited invasion into collagen gels.
    • Invasion was restored upon treatment with anti-adhesion peptides, confirming the role of desmosomal adhesion.

    Conclusions:

    • Desmosomal adhesion, mediated by desmocollin and desmoglein, is functionally important.
    • Desmosomal adhesion specifically inhibits cancer cell invasion in vitro.
    • These findings support the hypothesis that desmosomes play a role in suppressing tumor spreading and metastasis.