Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Processing and release of tumor necrosis factor alpha

N Watanabe1, K Nakada, Y Kobayashi

  • 1Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan.

European Journal of Biochemistry
|July 8, 1998
PubMed
Summary

Tumor necrosis factor alpha (TNFalpha) processing and release were studied in a novel cell line. Matrix metalloproteinases (MMPs) are key to TNFalpha release, with additional proteases involved in PMA-induced release.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Core outcome set for surgical trials in gastric cancer (GASTROS study): international patient and healthcare professional consensus.

The British journal of surgery·2021
Same author

Decrescent intensity training concurrently improves maximal anaerobic power, maximal accumulated oxygen deficit, and maximal oxygen uptake.

Physiology international·2019
Same author

Dramatic efficacy of cyclophosphamide pulsed therapy combined with double-filtration plasmapheresis for treating severe massive pulmonary hemorrhage associated with systemic lupus erythematosus.

Modern rheumatology·2014
Same author

Spike-monitoring of anaesthesia for corpus callosotomy using bilateral bispectral index.

Anaesthesia·2009
Same author

Paracetamol absorption test with Wagner-Nelson analysis for safe and accurate measurements of gastric emptying in women.

Methods and findings in experimental and clinical pharmacology·2009
Same author

An analog CMOS central pattern generator for interlimb coordination in quadruped locomotion.

IEEE transactions on neural networks·2008

Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Tumor necrosis factor alpha (TNFalpha) exists as a precursor, processed into a soluble form.
  • TNFalpha production and release are induced by activators like lipopolysaccharide in monocytes.

Purpose of the Study:

  • To investigate the mechanism of TNFalpha processing and release.
  • To establish a cell line for studying TNFalpha processing and release.

Main Methods:

  • Transfected NIH/3T3 cells with TNFalpha precursor cDNA to create a constitutively producing cell line.
  • Utilized phorbol 12-myristate 13-acetate (PMA) to augment TNFalpha processing and release.
  • Tested various protease inhibitors, including MMP inhibitors and specific chemical agents.

Related Experiment Videos

Main Results:

  • Matrix metalloproteinase (MMP) inhibitors blocked TNFalpha processing and release.
  • PMA-induced TNFalpha processing and release were mediated by a protein kinase C (PKC) pathway.
  • Specific inhibitors (3,4-dichloroisocoumarin, N alpha-p-tosyl-L-phenylalanine chloromethane, iodoacetamide, o-phenanthroline) inhibited PMA-induced release, suggesting roles for serine/cysteine proteases.

Conclusions:

  • MMPs are critically involved in the constitutive processing and release of TNFalpha.
  • PKC-mediated signaling pathways activate additional proteases for enhanced TNFalpha release.
  • The findings highlight the complex enzymatic machinery regulating TNFalpha bioavailability.