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Stability of adenosine in infusion
P Proot1, A Van Schepdael, A A Raymakers
1UZ Gasthuisberg Apotheek, Leuven, Belgium.
Journal of Pharmaceutical and Biomedical Analysis
|July 10, 1998
Summary
Adenosine stability in water was studied across various temperatures for 6 months. Results indicate adenosine is stable at room temperature for over 5 years, crucial for pharmaceutical applications.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Physical Chemistry
Background:
- Adenosine is a vital nucleoside with diverse biological roles.
- Understanding adenosine's stability is critical for its use in pharmaceuticals and research.
- Aqueous solutions can impact the long-term viability of sensitive biomolecules like adenosine.
Purpose of the Study:
- To determine the stability of adenosine in aqueous solution under various temperature conditions.
- To establish the shelf-life of adenosine at room temperature.
- To provide data for optimizing storage and handling protocols for adenosine.
Main Methods:
- Liquid chromatography was employed to quantify adenosine concentrations.
- Stability was assessed across five temperatures: 4, 22, 37, 60, and 72°C.
- Experiments were conducted over a 6-month period.
Main Results:
- Adenosine remained stable in aqueous solution at 4, 22, and 37°C throughout the 6-month study.
- Significant degradation of adenosine was observed at 60 and 72°C.
- The shelf-life (t90) at room temperature (22°C) was extrapolated to be a minimum of 5 years.
Conclusions:
- Adenosine exhibits excellent stability in aqueous solutions at typical storage temperatures.
- Elevated temperatures accelerate adenosine degradation, necessitating controlled storage conditions.
- The estimated 5-year shelf-life at room temperature supports its reliable use in various applications.