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Binding of murine leukemia virus Gag polyproteins to KIF4, a microtubule-based motor protein
1Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA.
Abstract:
A cDNA clone encoding a cellular protein that interacts with murine leukemia virus (MuLV) Gag proteins was isolated from a T-cell lymphoma library. The sequence of the clone is identical to the C terminus of a cellular protein, KIF4, a microtubule-associated motor protein that belongs to the kinesin superfamily. KIF4-MuLV Gag associations have been detected in vitro and in vivo in mammalian cells. We suggest that KIF4 could be involved in Gag polyprotein translocation from the cytoplasm to the cell membrane.
Insights
Researchers identified KIF4, a motor protein, interacting with murine leukemia virus (MuLV) Gag proteins. This interaction suggests KIF4 may facilitate the movement of viral proteins within cells.
Area of Science:
- Molecular Biology
- Virology
- Cell Biology
Background:
- Murine leukemia virus (MuLV) replication involves the Gag polyprotein.
- Cellular proteins play roles in viral replication processes.
- Microtubule-associated motor proteins, like kinesin, are crucial for intracellular transport.
Purpose of the Study:
- To identify cellular proteins that interact with MuLV Gag.
- To investigate the role of KIF4 in MuLV replication.
Main Methods:
- Isolation of a cDNA clone from a T-cell lymphoma library.
- Sequence analysis to identify the cellular protein.
- In vitro and in vivo association studies between KIF4 and MuLV Gag.
Main Results:
- A cDNA clone encoding a protein interacting with MuLV Gag was isolated.
- The identified protein was characterized as KIF4, a kinesin superfamily motor protein.
- KIF4 was shown to associate with MuLV Gag both in vitro and in vivo.
Conclusions:
- KIF4 interacts with MuLV Gag proteins.
- KIF4 may be involved in the translocation of Gag polyproteins from the cytoplasm to the cell membrane during MuLV replication.