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Turbidimetric determination of haptoglobin
A specific, accurate and simple determination of haptoglobin is described with an antiserum against the antigenic determinants of the three haptoglobin phenotypes 1-1, 2-1 and 2-2. Antihaptoglobin is the determining factor in the formation of an antigen-antibody complex with maximal light absorption properties. Haptoglobin concentration is expressed as hemoglobin binding capacity. For the three haptoglobin phenotypes we found similar correlation between the turbidimetric method and the direct measurement of hemoglobin binding capacity by agar-electrophoresis.
A specific, accurate and simple determination of haptoglobin is described with an antiserum against the antigenic determinants of the three haptoglobin phenotypes 1-1, 2-1 and 2-2. Antihaptoglobin is the determining factor in the formation of an antigen-antibody complex with maximal light absorption properties. Haptoglobin concentration is expressed as hemoglobin binding capacity. For the three haptoglobin phenotypes we found similar correlation between the turbidimetric method and the direct measurement of hemoglobin binding capacity by agar-electrophoresis.